{"product_id":"sigma-a-chymotrypsinogen-a-from-bovine-pancreas-essentially-salt-free-lyophilized-powder","title":"SIGMA a-Chymotrypsinogen A from bovine pancreas essentially salt-free, lyophilized powder","description":"\u003ch2\u003eSIGMA a-Chymotrypsinogen A from bovine pancreas essentially salt-free, lyophilized powder\u003c\/h2\u003e\n\u003cdiv class=\"MuiCollapse-root MuiCollapse-entered\"\u003e\n\u003cdiv class=\"MuiCollapse-wrapper\"\u003e\n\u003cdiv class=\"MuiCollapse-wrapperInner\"\u003e\n\u003cdiv class=\"jss286\"\u003e\n\u003cdiv class=\"MuiContainer-root MuiContainer-maxWidthLg\"\u003e\n\u003cdiv class=\"jss349\"\u003e\n\u003cdiv class=\"jss350\"\u003e\n\u003ch3 class=\"MuiTypography-root jss348 MuiTypography-h3\"\u003eGeneral description\u003c\/h3\u003e\n\u003cp class=\"MuiTypography-root jss351 MuiTypography-body1\"\u003eChymotrypsinogen from bovine pancreas is a zymogen containing 5 disulfide bridges. It has an isoelectric pH of 8.97.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cp class=\"jss350\"\u003eApplication - \u003cspan style=\"font-family: -apple-system, BlinkMacSystemFont, 'San Francisco', 'Segoe UI', Roboto, 'Helvetica Neue', sans-serif; font-size: 0.875rem;\"\u003eα-Chymotrypsinogen A from bovine pancreas has been used as model protein crystallization reproducibility studies.\u003c\/span\u003e\u003cspan style=\"font-family: -apple-system, BlinkMacSystemFont, 'San Francisco', 'Segoe UI', Roboto, 'Helvetica Neue', sans-serif; font-size: 0.875rem;\"\u003e \u003c\/span\u003e\u003cspan style=\"font-family: -apple-system, BlinkMacSystemFont, 'San Francisco', 'Segoe UI', Roboto, 'Helvetica Neue', sans-serif; font-size: 0.875rem;\"\u003eIt has also been used in the hydrolysis of α-gliadins prior to mass spectroscopy studies.\u003c\/span\u003e\u003c\/p\u003e\n\u003cdiv class=\"MuiTypography-root jss351 MuiTypography-body1\"\u003e\n\u003cp\u003eThe enzyme from Sigma has been used in the non-invasive determination of solid-state protein conformation using near infrared (NIR) spectroscopy.\u003cspan\u003e \u003c\/span\u003eIt has been used to study the partitioning of protein in polymer\/polymer aqueous two-phase systems.\u003c\/p\u003e\n\u003cp\u003eThe enzyme has also been used for self-interaction chromatography applications, to test the rapid measurement of protein osmotic second virial coefficients. In this technique, the protein is immobilized on chromatographic particles and its retention is measured using isocratic elution.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ch3 class=\"MuiTypography-root jss348 MuiTypography-h3\"\u003eBiochem\/physiol Actions\u003c\/h3\u003e\n\u003cdiv class=\"MuiTypography-root jss351 MuiTypography-body1\"\u003eChymotrypsinogen A requires limited proteolysis for its activation. Chymotrypsinogen A may be activated by trypsin and chymotrypsin (autolytic activation) to form m α, β, γ, δ and π chymotrypsin (depending upon the conditions of activation).\u003c\/div\u003e\n\u003cdiv class=\"MuiTypography-root jss351 MuiTypography-body1\"\u003e\u003c\/div\u003e\n\u003cdiv class=\"MuiTypography-root jss351 MuiTypography-body1\"\u003eChymotrypsin is a protease that will preferentially cleave peptides on the carboxyl side of aromatic amino acids including tryptophan, tyrosine, and phenylalanine. It will also hydrolyze peptides on the carboxyl side of leucine, methionine, and alanine.\u003c\/div\u003e\n\u003cdiv class=\"MuiTypography-root jss351 MuiTypography-body1\"\u003e\u003c\/div\u003e\n\u003cdiv class=\"MuiTypography-root jss351 MuiTypography-body1\"\u003eA serine protease that hydrolyzes peptide bonds with aromatic or large hydrophobic side chains (Tyr, Trp, Phe, Met) on the carboxyl end of the peptide bond.\u003c\/div\u003e\n\u003cdiv class=\"jss350\"\u003e\n\u003cp class=\"MuiTypography-root jss348 MuiTypography-h3\"\u003eUnit Definition\u003c\/p\u003e\n\u003cp class=\"MuiTypography-root jss351 MuiTypography-body1\"\u003eAfter activation to Chymotrypsin, one unit will hydrolyze 1.0 μmole of BTEE per min at pH 7.8 at 25 °C.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Sigma-Millipore","offers":[{"title":"Default Title","offer_id":45788386066501,"sku":"vex1789676767718","price":103.02,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0735\/9565\/0117\/files\/sigma-a-chymotrypsinogen-a-from-bovine-pancreas-essentially-salt-free-lyophilize_1.jpg?v=1789676776","url":"https:\/\/knotseamfit.shop\/products\/sigma-a-chymotrypsinogen-a-from-bovine-pancreas-essentially-salt-free-lyophilized-powder","provider":"My Store","version":"1.0","type":"link"}