{"product_id":"puresperm-buffer","title":"PureSperm Buffer","description":"\u003ch2\u003ePureSperm Buffer\u003c\/h2\u003e\n\u003cp\u003e\u003cb\u003eMaximize sperm survival during density gradient centrifugation. \u003c\/b\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003e\u003ci\u003ePureSperm Buffer\u003c\/i\u003e\u003c\/b\u003e is designed specifically for two functions; first as a balanced salt solution for diluting \u003cb\u003e\u003ci\u003ePureSperm 100\u003c\/i\u003e\u003c\/b\u003e\u003cb\u003e\u003ci\u003e \u003c\/i\u003e\u003c\/b\u003eto make up two layers of different densities for the gradient, and secondly, to reduce viscosity in the semen sample. The formula of \u003cb\u003e\u003ci\u003ePureSperm Buffer\u003c\/i\u003e\u003c\/b\u003e is optimized for maximum sperm survival  and greatest yield during density gradient centrifugation.\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003e\u003ci\u003ePureSperm Buffer\u003c\/i\u003e\u003c\/b\u003e\u003cspan\u003e is a sterile, isotonic, salt solution. It is optimised for the dilution of \u003c\/span\u003e\u003cb\u003e\u003ci\u003ePureSperm 100\u003c\/i\u003e\u003c\/b\u003e\u003cspan\u003e in the preparation of density gradients for the separation and purification of human sperm in ART. This gradient system effectively separates normal sperm from lymphocytes, epithelial cells, abnormal, immature and senescent sperm, cell debris, bacteria and seminal fluid.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003e\u003cspan\u003eWhy Use PureSperm Buffer?\u003c\/span\u003e\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eNow you can select the density layers they want to use, in any combination.\u003c\/li\u003e\n\u003cli\u003eThe same solution can be used to reduce viscosity in the semen sample.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eComponents\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eSodium chloride\u003c\/li\u003e\n\u003cli\u003eSodium citrate\u003c\/li\u003e\n\u003cli\u003ePotassium chloride\u003c\/li\u003e\n\u003cli\u003eHEPES\u003c\/li\u003e\n\u003cli\u003eCalcium lactate\u003c\/li\u003e\n\u003cli\u003eEDTA\u003c\/li\u003e\n\u003cli\u003eSodium pyruvate\u003c\/li\u003e\n\u003cli\u003eGlucose\u003c\/li\u003e\n\u003cli\u003ePurified water\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003ePerformance Characteristics\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003epH: 7.4-7.8.\u003c\/li\u003e\n\u003cli\u003eOsmolality (mOsm\/kg H2O): 300-310.\u003c\/li\u003e\n\u003cli\u003eEndotoxin levels: \u0026lt;1.0 EU\/mL.\u003c\/li\u003e\n\u003cli\u003eHuman sperm survival 18 hours after density gradient separation: \u0026gt;70%.\u003c\/li\u003e\n\u003cli\u003eContents are tested by human sperm survival.\u003c\/li\u003e\n\u003cli\u003eBottles and stoppers are M.E.A. tested.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eStorage and Stability\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eStore unopened bottles at 2 to 27ºC and avoid temperatures above or below these values. Under these conditions \u003cb\u003e\u003ci\u003ePureSperm Buffer\u003c\/i\u003e\u003c\/b\u003e has a shelf-life of 24 months. The expiry date is shown on both bottles and cartons.\u003c\/li\u003e\n\u003cli\u003eOpen and close bottles under aseptic conditions. After opening, store at 2 to 8ºC when not in use. Shelf-life on the product label applies when the product is stored according to manufacturer’s recommendations.\u003c\/li\u003e\n\u003cli\u003eNo antibiotics, unstable additives or preservatives have been added by the manufacturer to \u003cb\u003e\u003ci\u003ePureSperm Buffer\u003c\/i\u003e\u003c\/b\u003e.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003ePrecautions and Warnings\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eUse aseptic procedures at all times.\u003c\/li\u003e\n\u003cli\u003eIf available, use sealed centrifuge buckets during centrifugation to avoid creation of aerosols.\u003c\/li\u003e\n\u003cli\u003e\n\u003cb\u003e\u003ci\u003ePureSperm Buffer\u003c\/i\u003e\u003c\/b\u003e does not represent any fire or combustion hazard. A material safety data sheet is available from the distributor or manufacturer.\u003c\/li\u003e\n\u003cli\u003eDo not use any solution which shows evidence of bacterial contamination.\u003c\/li\u003e\n\u003cli\u003eDo not use contents if tamper-evident seal is broken.\u003c\/li\u003e\n\u003cli\u003eDo not re-use\u003c\/li\u003e\n\u003cli\u003eFederal Law (USA) restricts this device to sale by or on the order of a physician.\u003c\/li\u003e\n\u003cli\u003ePlease check for regulatory compliance governing the use of ART products in your country.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eGeneral Recommendations\u003c\/h3\u003e\n\u003cp\u003e\u003cspan\u003ePrepare two PureSperm gradients for each semen sample. This reduces the risk of overloading a single gradient, provides security when handling tubes or recovering sperm pellets, and provides two tubes to balance the centrifuge rotor.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003eRecommendations\u003cspan\u003e for a Viscous Sample\u003c\/span\u003e\n\u003c\/h3\u003e\n\u003cp\u003eIt can be extremely difficult to obtain a high yield from motile sperm from highly viscous semen samples. Nidacon has developed a method that could be helpful to you in the lab. You simply add PureSperm Buffer to the ejaculate, incubate at 37°C for 15-30 minutes and the sample is ready to use. This will give you a much higher proportion of motile sperm.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eProtocol: \u003c\/strong\u003e\u003cbr\u003e\u003cem\u003eFor sperm preparation from a viscous semen sample:\u003c\/em\u003e\u003c\/p\u003e\n\u003cp\u003e1. Bring all solutions to room temperature.\u003cbr\u003e2. Measure the volume of the semen sample.\u003cbr\u003e3. Dilute 1+3 , 1 part \u003cb\u003e\u003ci\u003ePureSperm Buffer\u003c\/i\u003e\u003c\/b\u003e and 3 parts semen sample\u003cbr\u003e(e.g. 0.5 ml \u003cb\u003e\u003ci\u003ePureSperm Buffer\u003c\/i\u003e\u003c\/b\u003e + 1.5 ml semen sample).\u003cbr\u003e4. Incubate at 37°C for 15-30 minutes.\u003cbr\u003e5. Mix using a pipette.\u003cbr\u003e6. Ready for sperm preparation on a density gradient.\u003c\/p\u003e\n\u003ch3\u003eDocuments\u003c\/h3\u003e\n\u003cp\u003eSDS – View PDF\u003cbr\u003eProduct insert (latest version) – View PDF\u003cbr\u003eLeaflet – View PDF\u003cbr\u003ePoster \u003cspan\u003e–\u003c\/span\u003e View PDF\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSpecifications:\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody style=\"font-size: 12px;\"\u003e\n\u003ctr\u003e\n\u003ctd\u003eMfr. No. \u003cspan\u003ePSB-100\u003c\/span\u003e\n\u003c\/td\u003e\n\u003ctd\u003ePureSperm Buffer \/ 100 mL (Not available in Europe)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"Nidacon","offers":[{"title":"1 EA","offer_id":45788311617605,"sku":"fgn1789674901156","price":57.42,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0735\/9565\/0117\/files\/puresperm-buffer_1.jpg?v=1789674909","url":"https:\/\/knotseamfit.shop\/products\/puresperm-buffer","provider":"My Store","version":"1.0","type":"link"}