{"product_id":"a-chymotrypsin-from-bovine-pancreas-type-ii-lyophilized-powder-40-unitsmg-protein","title":"a-Chymotrypsin from bovine pancreas Type II, lyophilized powder, =40 units\/mg protein","description":"\u003ch2 id=\"ctl00_ctl00_ctl00_BodyContent_BodyContent_BodyContent_ctl00_ptPageTitle_h1Heading\" class=\"heading heading--1\" data-source=\"u-miscellaneous-pagetitle\" data-mce-fragment=\"1\"\u003e\u003cspan id=\"ctl00_ctl00_ctl00_BodyContent_BodyContent_BodyContent_ctl00_ptPageTitle_spanItemProp\" data-mce-fragment=\"1\"\u003ea-Chymotrypsin from bovine pancreas Type II, lyophilized powder, =40 units\/mg protein\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eCAS Number\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003e9004-07-3\u003cbr\u003e\u003cstrong\u003eEC Number\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003e232-671-2\u003cbr\u003e\u003cstrong\u003eEnzyme Commission (EC)\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eNumber 3.4.21.1 ( BRENDA | IUBMB )\u003cbr\u003e\u003cstrong\u003eMDL number\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eMFCD00130481\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eAnalysis Note\u003c\/strong\u003e\u003cbr\u003eProtein determined by E1%\/280\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eBiochem\/physiol Actions\u003c\/strong\u003e\u003cbr\u003eA serine protease that hydrolyzes peptide bonds with aromatic or large hydrophobic side chains (Tyr, Trp, Phe, Met, Leu) on the carboxyl end of the bond.\u003c\/p\u003e\n\u003cp\u003eα-Chymotrypsin is a serine peptidase and has 241 amino acid residues contained in three polypeptide chains (A chain-13 residues, B chain-131 residues, and C chain-97 residues) linked by disulfide bridges. Molecular weight of this enzyme is found to be 25 kDa. The pI is 8.75. It selectively hydrolyzes peptide bonds on the C-terminal side of tyrosine, phenylalanine, tryptophan, and leucine. Ca2+ activates and stabilizes the enzyme. The enzyme is inhibited by diisopropyl fluorophosphate (DFP), phenylmethanesulfonyl fluoride (PMSF), N-p-tosyl-L-phenylalanine chloromethyl ketone (TPCK), chymostatin, aprotinin, α1-antitrypsin, and α2-macroglobulin, 10 mM Cu2+ and Hg2+.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003ePreparation Note\u003c\/strong\u003e\u003cbr\u003eDerived from New Zealand-sourced pancreas\u003c\/p\u003e\n\u003cp\u003eProduced from 3× crystallized chymotrypsinogen\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eUnit Definition\u003c\/strong\u003e\u003cbr\u003eOne unit will hydrolyze 1.0 μmole of BTEE per min at pH 7.8 at 25 °C.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eApplication\u003c\/strong\u003e\u003cbr\u003eThe enzyme from Sigma has been used to study the structure-function relationship in glycosylated α-chymotrypsin using immobilized metal-ion affinity chromatography (IMAC) and immobilized metal-ion affinity capillary electrophoresis (IMACE).\u003c\/p\u003e\n\u003ctable width=\"430\" class=\"base\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eQuality Level\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003ePREMIUM\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003etype\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eType II\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eform\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003elyophilized powder\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003emol wt\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003emol wt 25 kDa\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003ecomposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eprotein, ≥85%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003ecolor\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003ewhite to \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003estorage temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e−20°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGene Information\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003ecow ... CTRB1(618826)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"Millipore Sigma","offers":[{"title":"Default Title","offer_id":45788387573829,"sku":"xwj1789676796238","price":119.74,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0735\/9565\/0117\/files\/a-chymotrypsin-from-bovine-pancreas-type-ii-lyophilized-powder-40-unitsmg-protei_1.png?v=1789676806","url":"https:\/\/knotseamfit.shop\/products\/a-chymotrypsin-from-bovine-pancreas-type-ii-lyophilized-powder-40-unitsmg-protein","provider":"My Store","version":"1.0","type":"link"}